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    Bronwen Dekker said:

    Gosh! This brings back memories!

    I remember being rather nervous about contacting people to write, and then checking every five minutes to see whether they had responded. You told me: "Sometimes it is better that they take a little while to think it over before agreeing."

    There are some protocols that stand out for me (the others that I have worked on are also excellent, but some have special significance):

    The first person I invited to write was Chris Reutelingsperger:

    "In vitro measurement of cell death with the annexin A5 affinity assay":http://www.nature.com/nprot/journal/v1/n1/abs/nprot.2006.55.html

    The first time an image from a chemistry synthesis protocol was chosen for the cover was for "Volume 2, number 8":http://www.nature.com/nprot/journal/v2/n8/index.html

    ("The partial reduction of electron-deficient pyrroles: procedures describing both Birch (Li/NH3) and ammonia-free (Li/DBB) conditions":http://www.nature.com/nprot/journal/v2/n8/abs/nprot.2007.245.html)

    The first time I did all of the developmental editing for the protocol in notepad was for:

    "Locating proteins in the cell using TargetP, SignalP and related tools":http://www.nature.com/nprot/journal/v2/n4/full/nprot.2007.131.html

    And finally (at least for the moment)

    The protocol that stands out as having the largest number of figures (32) is:

    "SPIDER image processing for single-particle reconstruction of biological macromolecules from electron micrographs":http://www.nature.com/nprot/journal/v3/n12/abs/nprot.2008.156.html