Nature Methods | Methagora

Top downloads for July ’09

Two Correspondences made the list of top downloads for July coming in at #3 and #4, demonstrating that while this format may not report new methods it does have information of high interest to readers. The two top downloads seem to highlight a high level of interest in assaying single cells and using FRET to examine protein dynamics.

Top 8 research papers published in the July issue

1. Quantitative analysis of gene expression in a single cell by qPCR

2. Mapping the structure and conformational movements of proteins with transition metal ion FRET

3. Limitations and possibilities of small RNA digital gene expression profiling

4. Enabling IMAC purification of low abundance recombinant proteins from E. coli lysates

5. In vivo fluorescence imaging with high-resolution microlenses

6. Protein interaction platforms: visualization of interacting proteins in yeast

7. Agouti C57BL/6N embryonic stem cells for mouse genetic resources

8. Reaching the protein folding speed limit with large, sub-microsecond pressure jumps

There has been very little movement in the ten most popular papers published prior to our July issue and downloaded during July. The exception is the appearance of the HUPO test sample paper from June appearing at #7. The papers below this have changed but mostly because a large number of papers have very similar download numbers and they shuffle around from month to month.

Top 10 research papers published prior to the July issue

1. Mapping and quantifying mammalian transcriptomes by RNA-Seq

2. mRNA-Seq whole-transcriptome analysis of a single cell

3. Universal sample preparation method for proteome analysis

4. Stem cell transcriptome profiling via massive-scale mRNA sequencing

5. Isolation of human iPS cells using EOS lentiviral vectors to select for pluripotency

6. Genome-wide profiles of STAT1 DNA association using chromatin immunoprecipitation and massively parallel sequencing

7. A HUPO test sample study reveals common problems in mass spectrometry–based proteomics

8. Generation of transgene-free induced pluripotent mouse stem cells by the piggyBac transposon

9. Amplification-free Illumina sequencing-library preparation facilitates improved mapping and assembly of (G+C)-biased genomes

10. A versatile tool for conditional gene expression and knockdown

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