Inherently Responsive

Rapid Correspondence – Mitochondrial DNA polymorphism in birds

After reading with interest an article by Berlin et al. (Heredity 99, 389-396) on mitochondiral variability in birds, Anthony Hickey proposes an alternative interpretation to the data showing low mtDNA diversity, which they attributed to Hill-Robertson effects.


Paper by Berlin et al http://www.nature.com/hdy/journal/v99/n4/abs/6801014a.html

News and Commentary by G A B Marais http://www.nature.com/hdy/journal/v99/n4/full/6801034a.html

An alternate explanation for low mtDNA diversity in birds: an age-old solution?

Anthony J R Hickey

A recent article by Berlin et al. (2007) (also reviewed by Marais (Marais, 2007)) reported that the low mitochondrial variability in birds (relative to mammals) is most easily explained by Hill–Robertson effects on the W chromosome. These authors suggest selection and linkage between the W chromosome (in heterogametic females) and mtDNA act to lower avian mtDNA diversity. This may well be correct; however a well known biological phenomenon which appears unique to birds was excluded from these analyses, and may in fact provide a much simpler and more plausible explanation.

By comparison to mammals, birds are remarkable in several physiological parameters such as athletic performance, capacity to regenerate neuronal damage and their high respiratory efficiencies. However, perhaps more remarkable are several metabolic avian features. Birds have metabolic rates that are 2-2.5 fold greater, and estimated lifetime energy expenditures 15 times that of mammals of equivalent body mass. Not only do birds maintain body temperatures 3oC hotter than mammals, but many birds have blood glucose levels two to four times that of mammals, which in part defines them as diabetic (Holmes et al., 2001). This last feature occurs without the associated pathological complications seen in mammals and highlights considerable protection from oxidative damage.

With few exceptions, birds are also very long living relative to body mass, as many birds live three times longer, or more, than mammals of equivalent mass, and birds age slower at the cellular level (Holmes et al., 2001). Parrots may live over one hundred years, and even the tiny 5 gram Broad-billed hummingbird (Selasphorus platycercus) can live for 14 years (Holmes and Austad, 1995), while the maximum recorded life span of a 20 gram house mouse is only 4 years (Holmes et al., 2001). Zoo and wild tagging data also can mostly eliminate confounding influences of reduced predation through flight (Ricklefs, 1998), and the “exception species”, which are generally domesticated species, still live relatively long (e.g. chickens 20 years, Cortunix quail 6-7 years) (Holmes and Austad, 1995).

Just how birds achieve such exemplary resistance to age appears to be largely explained by different mitochondrial properties. In mammals 2-4% of all consumed oxygen is released as reactive oxygen species (ROS, e.g. superoxide O2-., hydroxyl radical OH-, hydrogen peroxide H2O2) from the electron transport system (ETS) complexes I and III. In health (and more so with numerous pathologies) mitochondria are generally the largest source of ROS (Turrens, 2003). Avian mitochondria produce considerably less ROS than mammalian mitochondria, with pigeon liver and heart mitochondria producing up to 10-fold less H2O2 than rats (this depends on respiration state, and ROS predominates from complexes I and III as O2-., which is converted to H2O2, (Barja, 2004; Herrero and Barja, 1997)). Furthermore, parrots and canaries show considerable resistance to lipid peroxidation relative to rodents, and isolated kidney epithelial cells from other long-lived bird species are much more resilient to pro-oxidant challenge by paraquat, H2O2 and 95% O2 with markedly less DNA damage than mouse cells (Ogburn et al., 1988). The differences between birds and mammals should not also be assumed to be adaptive. ROS release is not necessarily a byproduct of less efficient ETS function, as ROS provides feedback to cells and mitochondria (Barja, 2004), which explains why antioxidants can often be of detriment (Lane, 2005). These data do however illustrate increased ROS protection and lower ROS production in numerous bird species, which results in less DNA damage (Barja, 2004).

These avian physiological features were overlooked by Berlin et al. (2007). This is surprising given that the mitochondrial ETS is juxtaposed to mtDNA, and that ETS derived ROS makes the greatest contribution to mtDNA damage, and hence diversity (Barja, 2004). Admittedly ROS production would be a difficult parameter to measure for many species, although ROS production correlates more tightly with longevity than body mass (Barja, 2004). Therefore, correlation of π with mammalian and avian longevity may provide greater insight (note that this assumes the metabolic theory of ageing). Potentially the lower mitochondrial ROS output of bird mitochondria may provide another and potentially stronger physiological explanation for the low mtDNA diversity of birds.

AJR Hickey is at the School of Biological Sciences, University of Auckland, New Zealand.

email: a.hickey@auckland.ac.nz

Barja G (2004) Free radicals and ageing. Trends in Neurosciences 27, 3602-3607.

Berlin S, Tomaras D, Charlesworth B (2007) Low mitochondrial variability in birds may indicate Hill–Robertson effects on the W chromosome. Heredity 99, 389-396.

Herrero A, Barja G (1997) Sites and mechanisms responsible for the low rate of free radical production of heart mitochondria in the long lived pigeon. Mech. Age. Dev. 98, 95-111.

Holmes DJ, Austad SN (1995) Birds as models for the comparative biology of ageing: a prospectus. J. Gerontol. Biol. Sci. 50A, B59-B66.

Holmes DJ, Flückiger R, Austad SN (2001) Comparative biology of ageing in birds: an update. Exp. Gerontol. 36, 869-883.

Lane N (2005) Power, sex and suicide: mitochondria and the meaning of life. Oxford University Press New York

Marais GAB (2007) The Hill-Robertson effects extend from nucleus to mitochondria. Heredity 99, 357-358.

Ogburn CE, Austad SN, Holmes DJ, et al. (1988) Culture renal epithelial cells from birds and mice: enhanced resistance of avian cells to oxidative stress and DNA damage. J. Gerontol. Biol. Sci. 53A, B287-BB229.

Ricklefs RE (1998) Evolutionary theories of ageing: confirmation of a fundamental prediction, with implications for the genetic basis and evolution of life span. Am. Nat. 122, 22-44.

Turrens JF (2003) Mitochondrial formation of reactive oxygen species. J. Physiol. (Lond) 552, 335-344.

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